multiscribe rt kit Search Results


taqman microrna reverse transcription kit  (Thermo Fisher)
99
Thermo Fisher taqman microrna reverse transcription kit
Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/TaqMan+MicroRNA+Cells-to-CT+Kit/pmc05856835-50-15-20
Average 99 stars, based on 1 article reviews
taqman microrna reverse transcription kit - by Bioz Stars, 2026-09
99/100 stars
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multiscribe reverse transcriptase  (Qiagen)
97
Qiagen multiscribe reverse transcriptase
Multiscribe Reverse Transcriptase, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/Reverse+transcriptase/pmc04872073-37-16-9
Average 97 stars, based on 1 article reviews
multiscribe reverse transcriptase - by Bioz Stars, 2026-09
97/100 stars
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high capacity dna rt kit  (Thermo Fisher)
99
Thermo Fisher high capacity dna rt kit
High Capacity Dna Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/DNA/pm36009206-64-38-42
Average 99 stars, based on 1 article reviews
high capacity dna rt kit - by Bioz Stars, 2026-09
99/100 stars
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multiscribe reverse transcriptase kit  (Thermo Fisher)
99
Thermo Fisher multiscribe reverse transcriptase kit
Multiscribe Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/SUCROSE+EP%2FBP%2FNF+12KG/pmc05449153-247-12-16
Average 99 stars, based on 1 article reviews
multiscribe reverse transcriptase kit - by Bioz Stars, 2026-09
99/100 stars
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copy number variation tardbp mm00735064 cn  (Thermo Fisher)
88
Thermo Fisher copy number variation tardbp mm00735064 cn
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Copy Number Variation Tardbp Mm00735064 Cn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/Copy+Number+Variation+Tardbp%2C+Mm00735064_cn/pm34988393-245-131-130
Average 88 stars, based on 1 article reviews
copy number variation tardbp mm00735064 cn - by Bioz Stars, 2026-09
88/100 stars
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reverse transcriptase  (Thermo Fisher)
98
Thermo Fisher reverse transcriptase
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/Single+Cell-to-CT+qRT-PCR+Kit/pmc02891459-102-29-35
Average 98 stars, based on 1 article reviews
reverse transcriptase - by Bioz Stars, 2026-09
98/100 stars
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multiscribe mulv in a high capacity cdna kit  (Thermo Fisher)
95
Thermo Fisher multiscribe mulv in a high capacity cdna kit
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Multiscribe Mulv In A High Capacity Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/MagMAX+Microbiome+Ultra+Nucleic+Acid+Isolation+Kit%2C+with+bead+plate/pmc01592516-121-37-44
Average 95 stars, based on 1 article reviews
multiscribe mulv in a high capacity cdna kit - by Bioz Stars, 2026-09
95/100 stars
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rneasy kit  (Qiagen)
99
Qiagen rneasy kit
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/RNeasy+FFPE+Kit/pmc07892846-280-8-10
Average 99 stars, based on 1 article reviews
rneasy kit - by Bioz Stars, 2026-09
99/100 stars
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rneasy mini kit  (Qiagen)
99
Qiagen rneasy mini kit
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/RNeasy+Mini+Kit/pm22143988-87-6-9
Average 99 stars, based on 1 article reviews
rneasy mini kit - by Bioz Stars, 2026-09
99/100 stars
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rneasy micro plus kit  (Qiagen)
99
Qiagen rneasy micro plus kit
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Rneasy Micro Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/RNeasy+Plus+Micro+Kit/pmc08419171-31-5-9
Average 99 stars, based on 1 article reviews
rneasy micro plus kit - by Bioz Stars, 2026-09
99/100 stars
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hrv-1badvanced kit  (PrimerDesign Inc)
90
PrimerDesign Inc hrv-1badvanced kit
Figure 2. Genomic humanization of the endogenous mouse <t>Tardbp</t> gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.
Hrv 1badvanced Kit, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+rt+kit/hrv+1badvanced+kit/pm27726456-60-12-14
Average 90 stars, based on 1 article reviews
hrv-1badvanced kit - by Bioz Stars, 2026-09
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Image Search Results


Figure 2. Genomic humanization of the endogenous mouse Tardbp gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.

Journal: iScience

Article Title: Generation and analysis of innovative genomically humanized knockin SOD1 , TARDBP (TDP-43), and FUS mouse models.

doi: 10.1016/j.isci.2021.103463

Figure Lengend Snippet: Figure 2. Genomic humanization of the endogenous mouse Tardbp gene (A) A BAC construct, engineered to harbor the human TARDBP genomic sequence (blue) flanked by large regions of mouse homology (brown), was used as a donor to replace endogenous mouse Tardbp with human TARDBP in mouse ES cells via homologous recombination. (B) The humanized TARDBP allele in more detail, showing humanization of Tardbp from the ATG start codon to the TAG stop codon (blue). An FRT site in intron 3 is a remnant of selection cassette removal. (C–F) IGV visualizations of Oxford Nanopore read alignments via minimap2. Alignment of nanopore sequencing reads across (C) the mouse and human TARDBP gene loci, (D) the mouse and human TARDBP ATG start codon, (E) the mouse and human TARDBP stop codon, and (F) the wider TARDBP locus comparing B6 and 129 mouse strain identity, with dashed lines representing the boundaries of the homology arm region and dotted lines delineating B6 and 129 strain genome identity. Blue shading in alignment screenshots represents the humanized region. Black arrows in (D and E) show the precise transition from mouse-to-human and human-to-mouse sequence.

Article Snippet: Antibodies Anti TDP-43 (pan) Bio techne MAB7778 Anti TDP-43 (human specific) Proteintech 60019-2-Ig; RRID:AB_2200520 Anti TDP-43 (pan) Abcam ab104223; RRID:AB_10710019 GAPDH ThermoFisher AM4300; RRID:AB_2536381 GAPDH Proteintech 60004-1-Ig; RRID:AB_2107436 FUS 562 Novus NB100-562; RRID:AB_10002858 FUS 565 Novus NB100-565; RRID:AB_2105207 Anti SOD1 (pan) Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A Anti SOD1 (pan, native protein conformation) Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A IRDye 800CW Goat anti-Rabbit IgG (H+L) LI-COR 926-32211; RRID:AB_621843 SOD1 Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A Chemicals, peptides, and recombinant proteins Trizol reagent Life technologies 15596018 RIPA buffer ThermoFisher 89900 cOmplete Mini Protease Inhibitor Roche/Sigma 4693124001 Prolong Glass Antifade Mountant with NucBlue ThermoFisher P36981 Gallocyanine Sigma-Aldrich 124508-10G CV Ultra Mounting Media Leica 14070936261 Critical commercial assays Mouse Tardbp qPCR Taqman assay Mm00735064 ThermoFisher Mm00735064_cn Human TARDBP qPCR Taqman assay Hs06560655 ThermoFisher Hs06560655_cn RNeasy Lipid Tissue Mini Kit Qiagen 74804 RNeasy Fibrous Tissue Mini Kit Qiagen 74704 MultiScribe Reverse Transcriptase ThermoFisher 4311235 High Capacity cDNA Reverse Transcription Kit Applied biosystems 4368814 qScript cDNA Synthesis Quanta Bio 95047-500 qPCR Bio SyGreen mix LO-ROX PCR Biosystems PB20.11-05 Dream taq green PCR master mix (2X) Thermo Scientific K1081 Fast SYBR Green Master Mix ThermoFisher 4385610 DC Protein Assay BioRad 500-0116 REVERT Total Protein Stain Kit LI-COR 926-11010 Deposited data RNAseq fastq files deposited to NCBI Sequence Read Archive (SRA) https://www.ncbi.nlm.nih.gov/sra/PRJNA769952 SRA: PRJNA769952 Experimental models: Cell lines Mouse embryonic stem cell line R1 https://web.expasy.org/cellosaurus/CVCL_2167 RRID:CVCL_2167 Experimental models: Organisms/strains Humanised SOD1 mice: Sod1tm1.1(SOD1)Emcf This paper MGI:6403195; EM:13075 Humanised TARDBP mice: Tardbpem2.1(TARDBP)H This paper MGI:6513996, EM:14603 (Continued on next page) iScience 24, 103463, December 17, 2021 23

Techniques: Construct, Sequencing, Homologous Recombination, Selection, Nanopore Sequencing

Figure 5. hTARDBP/hTARDBP mice only express human TARDBP All tissues analyzed are from female 14-week-old hTARDBP mice and controls. (A) RT-PCR in brain using mouse-specific Tardbp and human-specific TARDBP primers. (B) Quantitative RT-PCR using conserved mouse-human Tardbp/TARDBP primers (n = 2–3 per genotype per tissue). (C) Quantitative RT-PCR using mouse Masp2 primers in liver (n = 3 per genotype). (D) Immunoblots using pan-mouse-human TDP-43 antibody (n = 2–3 per genotype). (E) Immunoblot using human-specific TDP-43 antibody in brain. **p< 0.01, ***p< 0.001, Mean G SD, One-way ANOVA with Dunnett’s post hoc test.

Journal: iScience

Article Title: Generation and analysis of innovative genomically humanized knockin SOD1 , TARDBP (TDP-43), and FUS mouse models.

doi: 10.1016/j.isci.2021.103463

Figure Lengend Snippet: Figure 5. hTARDBP/hTARDBP mice only express human TARDBP All tissues analyzed are from female 14-week-old hTARDBP mice and controls. (A) RT-PCR in brain using mouse-specific Tardbp and human-specific TARDBP primers. (B) Quantitative RT-PCR using conserved mouse-human Tardbp/TARDBP primers (n = 2–3 per genotype per tissue). (C) Quantitative RT-PCR using mouse Masp2 primers in liver (n = 3 per genotype). (D) Immunoblots using pan-mouse-human TDP-43 antibody (n = 2–3 per genotype). (E) Immunoblot using human-specific TDP-43 antibody in brain. **p< 0.01, ***p< 0.001, Mean G SD, One-way ANOVA with Dunnett’s post hoc test.

Article Snippet: Antibodies Anti TDP-43 (pan) Bio techne MAB7778 Anti TDP-43 (human specific) Proteintech 60019-2-Ig; RRID:AB_2200520 Anti TDP-43 (pan) Abcam ab104223; RRID:AB_10710019 GAPDH ThermoFisher AM4300; RRID:AB_2536381 GAPDH Proteintech 60004-1-Ig; RRID:AB_2107436 FUS 562 Novus NB100-562; RRID:AB_10002858 FUS 565 Novus NB100-565; RRID:AB_2105207 Anti SOD1 (pan) Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A Anti SOD1 (pan, native protein conformation) Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A IRDye 800CW Goat anti-Rabbit IgG (H+L) LI-COR 926-32211; RRID:AB_621843 SOD1 Professor Stefan Marklund, Medical Biosciences, Umeå University, Sweden N/A Chemicals, peptides, and recombinant proteins Trizol reagent Life technologies 15596018 RIPA buffer ThermoFisher 89900 cOmplete Mini Protease Inhibitor Roche/Sigma 4693124001 Prolong Glass Antifade Mountant with NucBlue ThermoFisher P36981 Gallocyanine Sigma-Aldrich 124508-10G CV Ultra Mounting Media Leica 14070936261 Critical commercial assays Mouse Tardbp qPCR Taqman assay Mm00735064 ThermoFisher Mm00735064_cn Human TARDBP qPCR Taqman assay Hs06560655 ThermoFisher Hs06560655_cn RNeasy Lipid Tissue Mini Kit Qiagen 74804 RNeasy Fibrous Tissue Mini Kit Qiagen 74704 MultiScribe Reverse Transcriptase ThermoFisher 4311235 High Capacity cDNA Reverse Transcription Kit Applied biosystems 4368814 qScript cDNA Synthesis Quanta Bio 95047-500 qPCR Bio SyGreen mix LO-ROX PCR Biosystems PB20.11-05 Dream taq green PCR master mix (2X) Thermo Scientific K1081 Fast SYBR Green Master Mix ThermoFisher 4385610 DC Protein Assay BioRad 500-0116 REVERT Total Protein Stain Kit LI-COR 926-11010 Deposited data RNAseq fastq files deposited to NCBI Sequence Read Archive (SRA) https://www.ncbi.nlm.nih.gov/sra/PRJNA769952 SRA: PRJNA769952 Experimental models: Cell lines Mouse embryonic stem cell line R1 https://web.expasy.org/cellosaurus/CVCL_2167 RRID:CVCL_2167 Experimental models: Organisms/strains Humanised SOD1 mice: Sod1tm1.1(SOD1)Emcf This paper MGI:6403195; EM:13075 Humanised TARDBP mice: Tardbpem2.1(TARDBP)H This paper MGI:6513996, EM:14603 (Continued on next page) iScience 24, 103463, December 17, 2021 23

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot